General Cell Based Assays
Eurofins Discoverx PathHunter Human LPA1 (EDG2) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human LPA1 (EDG2) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure EDG2 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human ADORA3 (A3) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADORA3 (A3) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADORA3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human DRD1 Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human DRD1 Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure DRD1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human RANKL (RANK) Signaling Reporter Cell-Based Assay Kit (U2OS)
The PathHunter Human RANKL (RANK) Signaling NF-kB Reporter Stable Cell Line Assay (U2OS) measures NF-kB pathway activation through detection of induced expression levels of a tagged reporter protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress CNR2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0183E2CP2M)
The cAMP Hunter CNR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR2, a naturally Gi/Go coupled GPCR. Upon activation of CNR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay, 2-plate Kit. (96-0046E1CP7M)
The InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter eXpress HRH2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0208E2CP0M)
The PathHunter HRH2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH2 and EA-tagged β-Arrestin-2. Activation of HRH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human GPR75 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR75 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR75 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human IL-6 (IL6RA/IL6ST) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-6 (IL6RA/IL6ST) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL6RA/IL6ST. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Sigma Aldrich Fine Chemicals Biosciences Mitochondrial Membrane Pot
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores leading to the release of cytochrome c into the cytosol which in turn triggers other downstream events in the apoptotic cascade.
Revvity Health Sciences Inc HTRF Total IR-β (Insulin Receptor Beta) Detection Kit, Control Lysate
HTRF Total IR-β (Insulin Receptor Beta) Detection Kit, Control Lysate
Eurofins Discoverx PathHunter Human NF-kB Pathway Reporter Cell-Based Assay Kit (HEK 293)
The PathHunter NF-kB Pathway Reporter Stable Cell Line Assay (HEK 293) monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR161 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR161 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR161 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress PTGER2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0214E2CP2M)
The PathHunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER2 and EA-tagged β-Arrestin-2. Activation of PTGER2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.